Available by default:
- Baby Mario
- Baby Peach
- Toad
- Koopa Troopa
- Mario
- Luigi
- Peach
- Yoshi
- Wario
- Waluigi
- Donkey Kong
- Bowser
Unlockable:
- Baby Luigi
- Baby Daisy
- Toadette
- Dry Bones
- Daisy
- Birdo
- Diddy Kong
- Bowser Jr.
- King Boo
- Rosalina
- Funky Kong
- Dry Bowser
Trek pilihan:
Wii Grand Prix:
Mushroom Cup:
- Luigi Circuit
- Moo Moo Meadows
- Mushroom Gorge
- Toad's Factory
Flower Cup:
- Mario Circuit
- Coconut Mall
- DK Summit
- Wario's Gold Mine
Star Cup:
- Daisy Circuit
- Koopa Cape
- Maple Treeway
- Grumble Volcano
Special Cup:
- Dry Dry Ruins
- Moonview Highway
- Bowser's Castle
- Rainbow Road
Retro Grand Prix:
Shell Cup:
- GCN Peach Beach
- DS Yoshi Falls
- SNES Ghost Valley 2
- N64 Mario Raceway
Banana Cup:
- N64 Sherbet Land
- GBA Shy Guy Beach
- DS Delfino Square
- GCN Waluigi Stadium
